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clontech smart seq ht kit  (TaKaRa)


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    Structured Review

    TaKaRa clontech smart seq ht kit
    Clontech Smart Seq Ht Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clontech+smart+seq+ht+kit/SMART-Seq+HT+Kit/pmc12693507-50-13-13
    Average 96 stars, based on 458 article reviews
    clontech smart seq ht kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Sequencing:

    Article Title: Platelet Gene Expression in Systemic Lupus Erythematosus and Cardiovascular Health
    Article Snippet: RNA quality and quantity were determined using an Agilent 2100 Bioanalyzer (Agilent Technologies). .. Sequencing libraries were generated with Clontech SMART-Seq HT kit (Takara Bio) for low-input mRNA per manufacturer’s instructions. .. The obtained libraries were quantified, normalized, pooled, and sequenced using the S1 100 flow cell on NovaSeq 6000 instrument (Illumina).

    Generated:

    Article Title: Platelet Gene Expression in Systemic Lupus Erythematosus and Cardiovascular Health
    Article Snippet: RNA quality and quantity were determined using an Agilent 2100 Bioanalyzer (Agilent Technologies). .. Sequencing libraries were generated with Clontech SMART-Seq HT kit (Takara Bio) for low-input mRNA per manufacturer’s instructions. .. The obtained libraries were quantified, normalized, pooled, and sequenced using the S1 100 flow cell on NovaSeq 6000 instrument (Illumina).

    Article Title: Promoting Biological Similarity by Collagen Microfibers in 3D Colorectal Cancer-Stromal Tissue: Replicating Mechanical Properties and Cancer Stem Cell Markers.
    Article Snippet: Total RNA was extracted using an miRNeasy Micro Kit (QIAGEN, Venlo, Netherlands). .. Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, USA). .. Each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA) according to the manufacturer’s instructions.

    Article Title: Direct relationship between protein expression and progeny yield of herpes simplex virus 1.
    Article Snippet: Next, 5 × 105 cells in the f1– f6 subpopulations were sorted into PBS by BD FACS Melody (Becton Dickinson). .. After centrifugation, total RNA from fractionated cells was isolated with a High Pure RNA Isolation kit (Roche). (i) Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA). ..

    Article Title: Direct relationship between protein expression and progeny yield of herpes simplex virus 1
    Article Snippet: Next, 5 × 10 5 cells in the f1–f6 subpopulations were sorted into PBS by BD FACS Melody (Becton Dickinson). .. After centrifugation, total RNA from fractionated cells was isolated with a High Pure RNA Isolation kit (Roche). (i) Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA). ..

    Article Title: A newly identified gene Ahed plays essential roles in murine haematopoiesis.
    Article Snippet: For RNA-Seq on E14.5 FL or BM LSK CD48− cells (Fig. 5), Total RNA was extracted from cells with an RNeasy Micro kit (Qiagen) following the manufacturer’s instruction. .. Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA). ..

    Synthesized:

    Article Title: Chromosome-Level Haplotype Resolved Genome Assembly of the Linguliform Brachiopod Discradisca antillarum (d'Orbigny, 1845).
    Article Snippet: RNA concentration was measured using a Thermo Fisher Qubit 3.0 12 fluorometer with the RNA High Sensitivity kit, purity was assessed by measuring the 260/280 13 nm absorbance ratio using a Thermo Fisher Nanodrop Lite, and integrity was evaluated using a 14 1% SB agarose gel. .. Complementary DNA (cDNA) was synthesized from 1 ng of total RNA using 15 the Clontech SMART-Seq HT kit. .. An Illumina sequencing library was then prepared in-house 16 using the Takara SMART-Seq HT Plus kit with 5 ng of cDNA.

    Article Title: Chromosome-level haplotype-resolved genome assembly of the linguliform brachiopod Discradisca antillarum (d'Orbigny, 1845)
    Article Snippet: RNA concentration was measured using a Thermo Fisher Qubit 3.0 fluorometer with the RNA High Sensitivity kit, purity was assessed by measuring the 260/280 nm absorbance ratio using a Thermo Fisher Nanodrop Lite, and integrity was evaluated using a 1% SB agarose gel. .. Complementary DNA (cDNA) was synthesized from 1 ng of total RNA using the Clontech SMART-Seq HT kit. .. An Illumina sequencing library was then prepared in-house using the Takara SMART-Seq HT Plus kit with 5 ng of cDNA.

    Centrifugation:

    Article Title: Direct relationship between protein expression and progeny yield of herpes simplex virus 1.
    Article Snippet: Next, 5 × 105 cells in the f1– f6 subpopulations were sorted into PBS by BD FACS Melody (Becton Dickinson). .. After centrifugation, total RNA from fractionated cells was isolated with a High Pure RNA Isolation kit (Roche). (i) Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA). ..

    Article Title: Direct relationship between protein expression and progeny yield of herpes simplex virus 1
    Article Snippet: Next, 5 × 10 5 cells in the f1–f6 subpopulations were sorted into PBS by BD FACS Melody (Becton Dickinson). .. After centrifugation, total RNA from fractionated cells was isolated with a High Pure RNA Isolation kit (Roche). (i) Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA). ..

    Isolation:

    Article Title: Direct relationship between protein expression and progeny yield of herpes simplex virus 1.
    Article Snippet: Next, 5 × 105 cells in the f1– f6 subpopulations were sorted into PBS by BD FACS Melody (Becton Dickinson). .. After centrifugation, total RNA from fractionated cells was isolated with a High Pure RNA Isolation kit (Roche). (i) Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA). ..

    Article Title: Direct relationship between protein expression and progeny yield of herpes simplex virus 1
    Article Snippet: Next, 5 × 10 5 cells in the f1–f6 subpopulations were sorted into PBS by BD FACS Melody (Becton Dickinson). .. After centrifugation, total RNA from fractionated cells was isolated with a High Pure RNA Isolation kit (Roche). (i) Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA). ..

    cDNA Synthesis:

    Article Title: Phylogenomics supports monophyly of marsupial crustaceans: a journey to direct development
    Article Snippet: RNA concentration was measured using a Thermo Fisher Qubit 3.0 fluorometer with the RNA High Sensitivity kit, purity was assessed by measuring the 260/280 nm absorbance ratio using a Thermo Fisher Nanodrop Lite, and integrity was evaluated using a 1% SB agarose gel. .. Complementary DNA (cDNA) synthesis was performed using 1 ng of total RNA using the Clontech SMART-Seq HT kit. .. An Illumina sequencing library was then prepared using either the Takara SMART-Seq HT Plus kit with 2-10 ng of cDNA or the Nextera XT kit with 0.125 ng of cDNA.



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